The bacterial community structure of anaerobic enrichment cultures that are capable of degrading both cis1,2dichloroethylene cisdce and vinyl chloride vc and isolation of the organism responsible for the degradation were investigated. Mycobacteriumchubuense strain nbb4 can grow on both alkanes and alkenes as carbon sources, and was hypothesised to be an effective bioremediation agent for chlorinated aliphatic pollutants. Both the cis and trans forms usually as a mixture are used as a solvent for waxes and resins. Stable carbon isotope fractionation during aerobic cdce biodegradation was observed in groundwater microcosms under varying incubation conditions room. A thorough understanding of halorespiration, also named organohalide respiration, was obtained 1,4,712. Epa, 2005a, there is inadequate information to assess the carcinogenic potential of cis 1, 2 dce. Thermophilic anaerobic biodegradation of tetrachloroethene pce was investigated with various inocula from geothermal and nongeothermal areas. Bmds modeling summary of relative kidney weight in female rats exposed to cis 1, 2 dce by gavage for 90 days. Page 2 of 43 1 preface 2 3 under the authority of the federal advisory committee act faca p. The most frequent uses of 1, 2 dichloroethene are solvent production and chemical mixtures 2300.
This study focuses on biodegradation of 1,2dichloroethane 1,2dca in micro. Breathing high levels of 1, 2 dichloroethene can make you feel nauseous, drowsy, and tired. Cometabolism of cis1,2dichloroethene by aerobic cultures grown on vinyl chloride as the primary substrate. Continued biodegradation of chloroethene compounds in. In this study, the ability of nbb4 to biodegrade vinyl chloride vc, cis dichloroethene cdce and 1, 2 dichloroethane dca was investigated under pureculture conditions and in microcosms. View products detecting gaseous dichloroethene, cis1,2 tvoc 2 fixed pid detector tvoc 2 is a fixed pid detector for continuous volatile organic compound voc monitoring with leading pid sensor technology and ultimate resistance to humidity and contamination.
Its primary uses are as an industrial solvent and as an intermediate to make other chemicals. The study is being conducted in three separate phases, the first to better understand the mechanisms involved in cdce and vc biodegradation, the second to evaluate the chemistry of the processes involved, and the third, to study factors. Health information summary 1,2dichloroethene 1,2dce is a synthetic chemical with no known natural sources. The odour thresholds for trans 1, 2 dichloroethene in air and water are 68 mgm3 and 0. Biodegradation of vinyl chloride, cis dichloroethene and. Cis1,2dichloroethylene is a highly flammable, colorless liquid with a sharp, harsh odor. Cis1,2dichloroethene has been detected in groundwater samples collected at of the 146 npl sites, in surface water samples collected at 14 of the 146 npl sites, and in leachate samples collected. Tetrachloroethene transformation to trichloroethene cis1,2. Although it exhibited the ability under feiiireducing conditions, the chlorinated ethenes degradationwas not linked to the feiii reduction. An aerobic bacterium capable of growth on cis dichloroethene cdce as a sole carbon and energy source was isolated by enrichment culture. Cis 1, 2 dichloroethylene is a highly flammable, colorless liquid with a sharp, harsh odor. Aerobic biodegradation of cis1,2dichloroethene as sole.
Performing organization names and addresses geosyntec consultants,31. The epa requires that any spills or accidental release of 1,000 pounds or. You can smell very small amounts of 1, 2dichloroethene in air about 17 parts of 1, 2dichloroethene per million parts of air 17 ppm. An anaerobic, feiiireducing enrichment culture, which originatedfrom a sediment sample collected at a landfill in nanjido, seoul, korea, was capable ofdegrading cis 1, 2 dichloroethylene cis dce and vinylchloride vc. J hata, k takamizawa, n miyata, k iwahoribiodegradation of cis 1, 2 dichloroethylene and vinyl chloride in anaerobic cultures enriched from landfill leachate sediment under feiiireducing conditions biodegradation, 14 2003, pp. Bmds modeling summary of decreased absolute thymus weight in female mice exposed to trans1,2dce in. Biodegradation of cisdichloroethene as the sole carbon. Carbon isotope fractionation during microbial dechlorination. Enhanced in situ biodegradation of trichloroethene1,1,1.
Biodegradation of cis1,2dichloroethene in simulated. A stall of cis1,2dce and vinyl chloride vc is frequently observed during. Biodegradation of vinyl chloride, cisdichloroethene and 1,2dichloroethane in the alkenealkaneoxidising mycobacterium strain nbb4. Tetrachloroethene transformation to trichloroethene cis1. Oct 06, 2004 read biodegradation of cis 1, 2 dichloroethylene and vinyl chloride in anaerobic cultures enriched from landfill leachate sediment under feiiireducing conditions, biodegradation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Stable carbon isotope fractionation in chlorinated ethene. Compared to the natural situation ns at a constant temperature of 10 c, both utes systems with 255 c for warm and cold well performed significantly better in cisdce cis1,2dichloroethene removal. Aerobic biodegradation of vinyl chloride and cis1,2dichloroethylene in aquifer sediments article in chemosphere 6011. Breathing high levels of 1,2dichloroethene can make you feel nauseous, drowsy, and tired. The odour thresholds for trans1,2dichloroethene in air and water are 68 mgm3 and 0. Aerobic biodegradation of the chloroethenes oxford academic. Js666 as a bioaugmentation agent for degradation of cis. The study is being conducted in three separate phases, the first to better understand the mechanisms involved in cdce and vc biodegradation, the second to evaluate the chemistry of the processes involved, and the third, to study factors affecting.
Under the guidelines for carcinogen risk assessment u. Stable carbon isotope analysis was performed with a gas chromatographcombustionisotoperatiomassspectrometry systemgccirms10,25. Here, we present the development and evaluation of a dna probe for polaromonas sp. You can smell very small amounts of 1,2dichloroethene in air about 17 parts of 1,2dichloroethene per million parts of air 17 ppm. Biodegradation of cis 1,2dichloroethylene and vinyl. Chloroethene concentration and ratios of trichloroethene to cis 1,2dichloroethene and cis 1,2dichloroethene to vinyl chloride concentrations at southern plantation sites mw14, p17, and p19 at operable unit 1, naval undersea warfare center, division keyport, washington, 19912004. Anaerobic microbial reductive dechlorination of tetrachloroethene to predominately trans1, 2dichloroethene.
Major uses 1,2dichloroethene cistrans mixture is used mainly as an intermediate in the synthesis of chlorinated solvents and compounds 5. Enhancing natural attenuation through bioaugmentation. Microcosm assessment of a dna probe applied to aerobic. Anaerobic degradation of cis1,2dichloroethylene and vinyl. It is used to produce solvents and in chemical mixtures. Tenant notification fact sheet for 1,2dichloroethene 1,2dce. Cis 1, 2 dichloroethylene is one of two forms of 1, 2 dichloroethylene also called 1, 2 dichloroethene. At 20c, strain js666 grew on cdce with a minimum doubling time of 73 7 h. Continued biodegradation of chloroethene compounds in ground. Hydrochemical conditions for aerobicanaerobic biodegradation of. Only polluted harbor sediment resulted in a stable enrichment culture that converted pce via trichloroethene to cis1,2dichloroethene at the optimum temperature of 60 to 65c.
Degradation of chlorinated organic compounds in general. Resting cells grown on cdce could transform cdce, ethene, vinyl chloride, trans dichloroethene, trichloroethene, and 1,2dichloroethane. Tenant notification fact sheet for 1,2dichloroethene 1,2. The 16s ribosomal dna sequence of the isolate strain js666 had 97. This threeyear project is to study the anaerobic biological conversion of cis1,2 dichloroethene cdce and vinyl chloride vc to ethene. The overall removal efficiency under mimicked ates and btes conditions were respectively and 8. Biodegradation of cis1,2dichloroethylene and vinyl chloride. Bioaugmentation for aerobic degradation of cis1,2dichloroethene.
Biodegradation of cisdichloroethene as the sole carbon source by. Cis1,2dichloroethene cdce is a compound of concern at many chloroethenecontaminated sites, since it tends to accumulate during reductive dechlorination of the higher chlorinated ethenes. Enhanced in situ biodegradation of trichloroethene1,1,1 trichloroethane in fractured rock groundwater. Exposure to 1,2dichloroethene may also result from exposure to other chemicals. Major uses 1, 2 dichloroethene cis trans mixture is used mainly as an intermediate in the synthesis of chlorinated solvents and compounds 5.
Aerobic biodegradation of vinyl chloride and cis1,2. The most frequent uses of 1,2dichloroethene are solvent production and chemical mixtures 2300. Sigmaaldrich offers a number of cis 1,2dichloroethene solution products. There is also a potential for release of 1,2dichloroethene to water from hazardous waste sites. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis1,2dichloroethene 5a.
It can exist as either of two geometric isomers, cis 1, 2 dichloroethene or trans 1, 2 dichloroethene, but is often used as a mixture of the two. In this study, the ability of nbb4 to biodegrade vinyl chloride vc, cisdichloroethene cdce and 1,2dichloroethane dca was investigated under pureculture. Microcosm assessment of a dna probe applied to aerobic degradation of cis1,2dichloroethene by polaromonas sp. A mixture of 1,2dichloroethene isomers has a pleasant odour 3.
Sigmaaldrich offers a number of cis 1,2dichloroethene products. Anaerobic degradation of cis1,2dichloroethylene and. Cometabolism of cis1,2dichloroethene by aerobic cultures. Explore information about air pollutants, contaminants and studies being done. Reductive dechlorination of tetrachloroethene to cis1,2. Technical information for trans1,2dichloroethene and cis1,2dichloroethene cas number. May 12, 2010 here, we present the development and evaluation of a dna probe for polaromonas sp. Exposure to 1,2dichloroethene occurs mainly in workplaces where it is made or used. Biodegradation mechanisms reductive dechlorination. Read biodegradation of cis1,2dichloroethylene and vinyl chloride in anaerobic cultures enriched from landfill leachate sediment under feiiireducing conditions, biodegradation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Denaturing gradient gel electrophoresis dgge of a pcramplified 16s rrna gene from the cultures showed the. It occurs in two forms known as cis and trans isomers that have similar properties.
However, tetrachloroethene wasdechlorinated to trichloroethene in 50mmbromoethanesulfonateinhibited enrichments and to trichloroethene and cis1,2dichloroethene in 3 mmfluoroacetateinhibited enrichments. It can exist as either of two geometric isomers, cis1,2dichloroethene or trans1,2dichloroethene, but is often used as a mixture of the two. Cis 1, 2 dichloroethene cdce is a compound of concern at many chloroethenecontaminated sites, since it tends to accumulate during reductive dechlorination of the higher chlorinated ethenes. Exposure to 1, 2 dichloroethene occurs mainly in workplaces where it is made or used. Epa, 2005a, there is inadequate information to assess the carcinogenic potential of cis1,2dce. Cometabolism of cis1,2dichloroethene by aerobic cultures grown. Pdf biodegradation of vinyl chloride, cisdichloroethene and 1,2. During the same period of time aerobic metabolic degradation of vc was extensively studied,14,15,16. Bmds modeling summary of decreased antibody directed against srbc in male mice exposed to trans1,2dce in drinking water for 90 days b14 b6. This may be a synopsis of the full weightofevidence narrative. A mixture of 1, 2 dichloroethene isomers has a pleasant odour 3. Extensive use and inadequate disposal of chloroethenes have led to. Substance details report page 2 of 4 synonym notation t statutesregulations sara 110 sara 110 sdwa npdwr sdwa npdwr tsca inv cis1,2dichloroethylene 1,2dichloroethene, ciscisdichloroethylene cis1,2dichloroethylene ethene, 1,2dichloro, 1zvalid valid valid valid. Biodegradation of vinyl chloride, cis dichloroethene and 1,2.
Sequential anaerobicaerobic biodegradation of chloroethenesa. Download citation aerobic biodegradation of vinyl chloride and cis1,2 dichloroethylene in aquifer sediments laboratory batch experiments have been. As there is currently no evidence for aerobic biodegradation of. However, tetrachloroethene wasdechlorinated to trichloroethene in 50mmbromoethanesulfonateinhibited enrichments and to trichloroethene and cis 1, 2 dichloroethene in 3 mmfluoroacetateinhibited enrichments. An anaerobic, feiiireducing enrichment culture, which originatedfrom a sediment sample collected at a landfill in nanjido, seoul, korea, was capable ofdegrading cis1,2dichloroethylene cisdce and vinylchloride vc.
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